Zymoseptoria ardabiliae YMS RNA sequencing - SRA

SRX894063: Zymoseptoria ardabiliae YMS RNA sequencing
1 ILLUMINA (Illumina HiSeq 2000) run: 27.4M spots, 1.4G bases, 858.9Mb downloads

Design: Total RNA was extracted from fungal cells grown in YMS (4 g yeast extract, 4 g malt extract, 4 g sucrose, 20 g bacto agar, 1 l H2O) agar in a shaking incubator at 200rpm at 18°C using the TRIZOL reagent (Invitrogen, Darmstadt, Germany) following the manufacturers protocol. Illumina RNA-seq libraries for two axenic culture replicates per species were prepared from an input of 10 µg total purified polyA RNA. Libraries were quantified by fluorometry, immobilized, and processed onto a flow cell with a cBot (Illumina) followed by sequencing-by-synthesis with TruSeq v3 chemistry on a HiSeq2000 at the Max Planck Genome Center (Cologne, Germany)

Submitted by: Christian-Albrechts University of Kiel

Study: Zymoseptoria ardabiliae STIR04_1.1.1 Transcriptome or Gene expression

PRJNA277174 • SRP055869 • All experiments • All runs

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Reconstructed transcripts from RNA sequencing data.

Sample:

SAMN02981326 • SRS860073 • All experiments • All runs

Organism: Zymoseptoria ardabiliae STIR04_1.1.1

Library:

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: PolyA

Layout: SINGLE

Spot descriptor:

1 forward

Runs: 1 run, 27.4M spots, 1.4G bases, 858.9Mb

Run	# of Spots	# of Bases	Size	Published
SRR1822499	27,410,581	1.4G	858.9Mb	2015-08-01

ID:: 1285414

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